Intestinal Epithelial Cell Surface Membrane Glycoprotein Synthesis

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Intestinal Epithelial Cell Surface Membrane Glycoprotein Synthesis

The cell surface membranes of mitotically active crypt cells from rat intestinal epithelium were found to have glycosyltransferase activities and endogenous acceptors not found on the differentiating villus cells. Rat intestinal epithelial cells were isolated as intact cells from different levels of the villus and crypt areas representing sequential stages of cellular differentiation. Incubatio...

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Characterization of a 180-kDa Intestinal Epithelial Cell Membrane

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Synthesis of plasmalemmal glycoproteins in intestinal epithelial cells. Separation of Golgi membranes from villus and crypt cell surface membranes; glycosyltransferase activity of surface membrane

The relationship between Golgi and cell surface membranes of intestinal cells was studied. These membranes were isolated from intestinal crypt cells and villus cells. The villus cell membranes consisted of microvillus membrane, a Golgi-rich fraction, and two membrane fractions interpreted as representing lateral-basal membranes. The villus cell microvillus membrane was purified by previously pu...

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The intestinal epithelial cell.

1 SHOULD first like to thank you for inviting me to be the Robert Campbell Orator on this occasion. For anyone in the field of medical science it is a great honour to give this lecture, for a Queen's graduate it is a very special honour, but for a Queen's graduate who is also an exile, it is not only an honour but a very welcome opportunity to come back again to this medical school. Although my...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1973

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(19)44142-2